PATTERN OF GENE MUTATIONS IN RESISTANT MYCOBACTERIUM TUBERCULOSIS STRAINS USING THE GENOTYPE MTBDRPLUS ASSAY

Ado Umar Adamu, Dalha Wada Taura, Muhammad Dauda Mukhtar, Salwa Shehu Dawaki, Abubakar Isiyaku, Umar Faruq Abubaka

Abstract


The rise of multidrug-resistant (MDR) Mycobacterium tuberculosis strains complicates tuberculosis (TB) treatment, necessitating rapid detection methods for drug resistance mutations. This study assessed gene mutation patterns in MDR, isoniazid (INH) monoresistant, and rifampicin (RIF) monoresistant MTB strains using the Genotype MTBDRplus assay, targeting mutations in the rpoB, katG, and inhA genes(Hillemann et al., 2007). Among the RIF-resistant MDR strains, mutations were most frequent at the rpoB WT8 probe (85.4%), followed by WT5 (12.2%), WT4 (9.8%), and WT3 (7.3%). For INH-resistant strains, 68.3% of MDR cases showed mutations due to lack of binding at the katG WT probe, while 73.2% had the S315T1 mutation (MUT1), a major marker for INH resistance. The S531T2 mutation was absent in MDR strains. Additionally, in INH monoresistant strains, 33.3% displayed mutations at the katG WT probe and S315T1 mutation. The inhA gene mutations in MDR strains included WT1 (-15/-16) at 19.5% and C15T (MUT1) at 17.1%. This study confirms the effectiveness of the Genotype MTBDRplus assay in detecting resistance patterns and highlights key mutation frequencies, informing targeted treatment approaches in TB control.


Keywords


Mycobacterium tuberculosis, MDR-TB, drug resistance, Genotype MTBDRplus assay, gene mutations, rpoB, katG, inhA, rifampicin, isoniazid

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