BACTERIOLOGICAL EXAMINATION OF OIL CONTAMINATED SOIL FROM AUTOMOBILE WORKSHOPS AT OBA, ANAMBRA STATE, NIGERIA
Abstract
Automobile repair is a lucrative economic activity found across different cities across Nigeria. One of the major challenges that come with this business is unregulated allocation of these workshops and consequent oil pollution of the soil and surrounding environment. This study aimed at evaluating bacterial communities found in soil samples from automobile shops located at Oba, Anambra State. A 10 g portion of each soil samples (n=5) were examined using 1 in ten-fold serial dilution, culturing on nutrient agar, and characterizing the isolates using Gram stain and other biochemical procedures. Microbial counts were noted as well as percentage occurrence of isolates in each soil sample. Hydrocarbon degraders were also isolated using the vapour-phase transfer method. Staphylococcus, Bacillus, Pseudomonas, Streptococcus and Enterobacter species were identified. Soil samples from automobile sites B and E had the highest bacterial counts of 7.3 x 103 cfu/g, while Bacillus and Staphylococcus species were the most occurring isolates with occurrence of 100%. The vapour phase transfer method showed the presence of Rhodococcus, Campylobacter, Bacillus, Alcaligenes and Pseudomonas species as the hydrocarbon degraders present in the examined soil samples. This study thus showed that oil polluted soils from these mechanic workshops contain bacterial communities that can possibly biodegrade them through natural attenuation, if these sites are left to fallow for a given period of time.
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